The overarching goal of this CEGS is to interpret how human genomic variations affect local and global properties of cellular networks to induce disease, using viral perturbations as a surrogate for genetic perturbations of network structure
- Marc Vidal and David Hill
Center for Cancer Systems Biology, Dana-Farber Cancer Institute
The goals of CCSB are to understand how macromolecular networks control biological processes and accordingly how perturbations in such networks can explain human disease. Within the CEGS the role of the CCSB is to map and interpret viral-host binary interactions. Also, as the central CEGS PI Dr. Vidal coordinates the efforts of all other CEGS investigators.
Vidal M, Cusick ME & Barabási AL, Interactome networks and human disease. Cell 2011
- Albert-László Barabási Center for Complex Network Research, Northeastern University
The researchers at the CCNR investigate how networks emerge, what networks look like, how networks evolve, and how networks provide explanations into the behavior of complex systems. They contribute to the CEGS by leveraging their expertise in network analysis to provide insight into the properties of viral-host interactome networks.
Goh et al, The human disease network. Proc Natl Acad Sci USA 2007
- James Decaprio Dept of Medical Oncology, Dana-Farber Cancer Institute
The DeCaprio laboratory is focused on understanding the mechanisms of cellular transformation by oncogenic viral proteins, in particular, simian virus 40 and related polyomaviruses. Their expertise contributes to the CEGS by discovering and interpreting polyomaviral-host and adenoviral-host interactions.
DeCaprio JA, How the Rb tumor suppressor structure and function was revealed by the study of Adenovirus and SV40. Virology 2009
- Elliott Kieff Channing Laboratory, Brigham and Women’s Hospital
The viral oncology group led by Dr. Kieff investigates cellular infection by oncogenic viruses, Epstein-Barr virus (EBV) in particular. EBV infection has been associated with several cancers: Burkitt lymphoma, Hodgkin lymphoma, and nasopharyngeal carcinoma. This group contributes to the CEGS by discovering and interpreting EBV-host interactions.
Calderwood et al, Epstein-Barr virus and virus human protein interaction maps. Proc Natl Acad Sci USA 2007
- Jarrod Marto Blais Proteomics Center, Dana-Farber Cancer Institute
The Blais Proteomics Center directed by Dr. Marto has wide expertise in the use of mass spectrometry-based proteomics for high-throughput, unbiased identification and characterization of proteins in biological systems. Within the CEGS they apply this expertise to the identification and interpretation of viral-host co-complex associations.
Adelmant G & Marto JA, Protein complexes: the forest and the trees. Expert Rev Proteomics 2009
- Karl Münger Channing Laboratory, Brigham and Women’s Hospital
Research in the Münger group is centered on understanding the roles of disparate human papillomaviruses (HPV) in cervical cancer, with particular focus on the HPV oncoproteins E6 and E7. The contribution of this group within the CEGS is discovery and interpretation of papillomaviral-host interactions.
McLaughlin-Drubin ME & Münger K, Oncogenic activities of human papillomaviruses. Virus Res 2009
- John Quackenbush Center for Cancer Computational Biology, Dana-Farber Cancer Institute
The research at the CCCB is dedicated to developing new methods to improve analysis and interpretation of genomic data through integration of diverse data types. Within the CEGS they apply this expertise to transcriptome profiling of perturbations of host cells brought about by viral protein expression.
Zhao B et al, Epstein-Barr virus nuclear antigen 3C regulated genes in lymphoblastoid cell lines. Proc Natl Acad Sci USA 2011
- Frederick P. (Fritz) Roth Donnelly Centre for Cellular & Biomolecular Research,
University of Toronto
The computational and experimental biology groups led by Dr. Roth share the goal of designing and interpreting large-scale experiments to comprehend pathway structure and its relationship to phenotype and human disease. Their expertise in all aspects of computational biology contributes to the CEGS by providing insight into the biological function and disease associations of host proteins targeted by viral proteins.
Tasan M et al, A resource of quantitative functional annotation for Homo sapiens genes. Genes Genomes Genet 2012
- Yves Jacob (Auxiliary CEGS Investigator) Unit of Genetics, Papillomavirus and Human Cancer, Pasteur Institute, Paris, France
Dr. Jacob has joined the CEGS project at CCSB for his two-year sabbatical from the Pasteur Institute. He brings his expertise and experience in developing higher sensitivity assays for detection of protein interactions, particularly between human papillomavirus (HPV) proteins and host proteins.
Cassonnet P et al, Benchmarking a luciferase complementation assay for detecting protein complexes. Nat Methods 2011